1209 The fate of melanocytes and the disorganization of basement membrane in a guinea pig model of rhododendrol-induced chemical vitiligo

Rhododendrol (RD) is a phenolic compound that was first developed as skin-lightening agent occasionally induces skin leukoderma. Although it has been shown RD melanocyte death in vitro, still not fully understood why melanocytes are gone by vivo. This research aimed to investigate how eliminated the animal model RD. On backs of black guinea pigs (JY-4) with epidermal basal layer, 30% administered topically three times per day, five days week. day 21, L* values (brightness index) RD-applied area were significantly higher than vehicle control area. 14, already induced obvious depigmentation several animals. From 1 7, number TRP1-positive and melanin layer decreased, but no TUNEL-positive identified. other hand, an accumulation newly found dermis. Immunohistochemical staining identified upper dermis or spinous away from basement membrane. An investigation epidermal-dermal interface using electron microscopy showed structural anomaly portion shows aberrant membrane structure might be responsible for detachment decrease melanocytes, which cause depigmentation. vivo gives different perspective vitrofindings may used elucidate novel mechanism action only RD-induced leukoderma also vitiligo, fate lesional should further investigated.